Collagen breaks at weak sacrificial bonds taming its mechanoradicals

Abstract:

Collagen is a force-bearing, hierarchical structural protein important to all connective tissue. In tendon collagen, high load even below macroscopic failure level creates mechanoradicals by homolytic bond scission, similar to polymers. The location and type of initial rupture sites critically decide on both the mechanical and chemical impact of these micro-ruptures on the tissue, but are yet to be explored. We here use scale-bridging simulations supported by gel electrophoresis and mass spectrometry to determine breakage points in collagen. We find collagen crosslinks, as opposed to the backbone, to harbor the weakest bonds, with one particular bond in trivalent crosslinks as the most dominant rupture site. We identify this bond as sacrificial, rupturing prior to other bonds while maintaining the material’s integrity. Also, collagen’s weak bonds funnel ruptures such that the potentially harmful mechanoradicals are readily stabilized. Our results suggest this unique failure mode of collagen to be tailored towards combatting an early onset of macroscopic failure and material ageing.

SEEK ID: https://publications.h-its.org/publications/1540

DOI: 10.1101/2022.10.17.512491

Projects: Molecular Biomechanics

Publication type: Journal

Journal: biorxiv

Citation:

Date Published: 18th Oct 2022

URL:

Registered Mode: manually

Authors: Benedikt Rennekamp, Christoph Karfusehr, Markus Kurth, Aysecan Ünal, Kai Riedmiller, Ganna Gryn’ova, David M. Hudson, Frauke Gräter

Help
help Submitter
Citation
Rennekamp, B., Karfusehr, C., Kurth, M., Ünal, A., Riedmiller, K., Gryn’ova, G., Hudson, D. M., & Gräter, F. (2022). Collagen breaks at weak sacrificial bonds taming its mechanoradicals. In []. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2022.10.17.512491
Activity

Views: 149

Created: 21st Oct 2022 at 17:53

Last updated: 25th Oct 2022 at 14:12

help Tags

This item has not yet been tagged.

help Attributions

None

Related items

Powered by
(v.1.11.0)
Copyright © 2008 - 2021 The University of Manchester and HITS gGmbH

This website uses essential cookies to keep your session open and to save if you accept this banner.

By continuing to use this site you agree to the use of cookies