Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Abstract:

iscovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. The first structure in nascent centrioles is a cartwheel, which comprises stacked 9-fold symmetrical SAS-6 ring polymers emerging orthogonal to a surface surrounding each resident centriole. The mechanisms through which SAS-6 polymerization ensures centriole organelle architecture remain elusive. We deploy photothermally-actuated off-resonance tapping high-speed atomic force microscopy to decipher surface SAS-6 self-assembly mechanisms. We show that the surface shifts the reaction equilibrium by ~104 compared to solution. Moreover, coarse-grained molecular dynamics and atomic force microscopy reveal that the surface converts the inherent helical propensity of SAS-6 polymers into 9-fold rings with residual asymmetry, which may guide ring stacking and impart chiral features to centrioles and cilia. Overall, our work reveals fundamental design principles governing centriole assembly.

SEEK ID: https://publications.h-its.org/publications/1360

PubMed ID: 34702818

DOI: 10.1038/s41467-021-26329-1

Research Groups: Molecular Biomechanics

Publication type: Journal

Journal: Nature Communications

Publisher: Springer Science and Business Media LLC

Citation: Nat Commun,12(1):6180.

Date Published: 26th Oct 2021

URL:

Registered Mode: manually

Authors: Niccolò Banterle, Adrian P. Nievergelt, Svenja de Buhr, Georgios N. Hatzopoulos, Charlène Brillard, Santiago Andany, Tania Hübscher, Frieda A. Sorgenfrei, Ulrich S. Schwarz, Frauke Gräter, Georg E. Fantner, Pierre Gönczy

help Submitter
Citation
Banterle, N., Nievergelt, A. P., de Buhr, S., Hatzopoulos, G. N., Brillard, C., Andany, S., Hübscher, T., Sorgenfrei, F. A., Schwarz, U. S., Gräter, F., Fantner, G. E., & Gönczy, P. (2021). Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture. In Nature Communications (Vol. 12, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/s41467-021-26329-1
Activity

Views: 4001

Created: 3rd Dec 2021 at 07:15

Last updated: 5th Mar 2024 at 21:24

help Tags

This item has not yet been tagged.

help Attributions

None

Powered by
(v.1.14.2)
Copyright © 2008 - 2023 The University of Manchester and HITS gGmbH